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KMID : 0604520170430010035
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Journal of the Society of Cosmetic Scientists of Korea
2017 Volume.43 No. 1 p.35 ~ p.41
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Anti-melanogenesis Active Constituents from the Extracts of Carpinus turczaninowii Leaves
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Kang Ji-Mi
Kim Jung-Eun
Lee Nam-Ho
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Abstract
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Melanin synthesis inhibition activities were investigated for the extracts prepared from the leaves of Carpinus turczaninowii (C. turczaninowii) by using B16F10 melanoma cells. As a result, the ethanol extract (100 μg/mL) showed 72.2% inhibition activities without cell toxicities in MTT assays. For the solvent fractions (n-hexane, ethyl acetate, n-butanol, water), the most potent activities were observed at the ethyl acetate fraction. To isolate the active constituents, the ethyl acetate fraction was further purified to afford four compounds; ethyl gallate (1), quercetin rhamnose (2), kaempferol rhamnose (3) and quercetin galloylrhamnose (4). The identification of the isolates was made by spectroscopic data including NMR spectra, and all of the compounds 1-4 were isolated for the first time from the leaves of C. turczaninowii. Anti-melanogenesis activities were studied for the isolates 1-4, and the compound 4 was determined to decrease the melanin synthesis dose-dependently without causing cell toxicities. ELISA measurement indicated that the isolate 4 decreased the contents of cell tyrosinase, a critical enzyme in melanogenesis. Based on these results, the extracts of C. turczaninowii were found to be applicable as whitening ingredients in cosmetic formulations.
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KEYWORD
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Carpinus turczaninowii , anti-melanogenesis , B16 melanoma cells , constituents
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